MagMAX™-96 Total RNA Isolation Kit

RNA isolation / purification Tissue - Human Duodenum

Experiment
RNA isolation / purification Tissue - Human Duodenum
Product
MagMAX™-96 Total RNA Isolation Kit from Thermo Fisher Scientific
Manufacturer
Thermo Fisher Scientific

Protocol tips

Upstream tips
- This kit can be used for the isolation of RNA from ≤ 5 mg animal tissue.
Downstream tips
- To avoid DNA contamination, either reduce sample size, or include an additional DNase treatment step after RNA isolation

Publication protocol

NA from each sample was isolated using MagMAX-96 Total RNA (AM1830; Applied Biosystems), RNA isolation kits, and used as input for library generation with Takara SMARTer Stranded Total RNA Sample Prep Kit (634876; Takara Bio USA). A total of 26 samples were sequenced for 50-bp single-end reads across 10 lanes on an Illumina HiSeq 2500 by the University of Michigan DNA Sequencing Core. All reads were quantified using Kallisto pseudo alignment to an index of transcripts from all human genes within the Ensembl GRCh38 database.12 Gene level data then were generated from the Kallisto estimated counts per transcript using the Bioconductor package tximport.13 Differential expression analysis was performed using the Bioconductor package DESeq2 and the gene level count data. A gene was considered to be differentially expressed if it had a 2-fold or larger difference between groups and an adjusted P value of .01 or less. Principal component analysis and sample clustering were performed in R with log2 transformed and centered gene counts of gene level data on all genes that had a sum of at least 10 counts across all samples. Replicates for all samples were clustered by Euclidian distance, and pairwise Pearson correlation coefficients were plotted in R. All reads are deposited at the EMBL-EBI ArrayExpress archive under accession E-MTAB-6516.

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Discussion

Discussion

1 year ago

Author: Paul G. Macon United States

RNA isolation from tissue

How do I extract RNA from animal tissue without using liquid nitrogen? I tried the RNA extraction by using the TRIzol reagent and I homogenize the tissue using polytron homogenizer at room temperature for 30secs is this correct?

Discussion

2 years ago

Author: Aaron Stege Netherlands

Problem in phase separation after using serum/plasma kit

I used a serum/plasma kit for my serum samples. After the phase separation the samples should have 3 phases: a colourless aqueous phase, a white interphase and a red organic phase. However, in some of my samples there was no aqueous phase unless I wait for an extended period of time. How can I circumvent this problem?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Tissue - Human Duodenum using MagMAX™-96 Total RNA Isolation Kit from Thermo Fisher Scientific.

Paper title
A Method for Cryogenic Preservation of Human Biopsy Specimens and Subsequent Organoid Culture
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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for MagMAX™-96 Total RNA Isolation Kit below.

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