RNeasy Fibrous Tissue Mini Kit

RNA isolation / purification Tissue - Human Joints

Experiment
RNA isolation / purification Tissue - Human Joints
Product
RNeasy Fibrous Tissue Mini Kit from Qiagen
Manufacturer
Qiagen

Protocol tips

Upstream tips
- This kit purifies RNA from up to 30 mg tissue
Downstream tips
- If there is low RNA yield,Repeat RNA elution, incubate the RNeasy Mini spin column on the benchtop for 10 min with RNase-free water before centrifuging.

- If there is low Low A260/A280 value use 10 mM Tris·Cl, pH 7.5, not RNase-free water, to dilute the sample before measuring purity.

- If RNA does not perform well in downstream experiments this could be due to salt carryover dyuring elution step, so ensure that Buffer RPE is at 15–25°C.

Publication protocol

Non-frozen and thawed synovial biopsies were cultured for 24 hours and subsequently snap-frozen in liquid nitrogen. Total RNA was isolated using the RNeasy fibrous tissue kit (Qiagen, Venlo, The Netherlands). Frozen synovial samples were transferred to MagNA Lyser Green Beads tubes (Roche) containing RLT lysis buffer. The tissue was disrupted in the MagNA lyser for 3 x 20 sec at 6000 rpm with one minute cooling between cycles. The remainder of the procedure was performed according to manufacturer instructions with on column DNA digestion.

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Discussion

Discussion

4 years ago

Author: Paul G. Macon United States

RNA isolation from tissue

How do I extract RNA from animal tissue without using liquid nitrogen? I tried the RNA extraction by using the TRIzol reagent and I homogenize the tissue using polytron homogenizer at room temperature for 30secs is this correct?

Discussion

4 years ago

Author: Aaron Stege Netherlands

Problem in phase separation after using serum/plasma kit

I used a serum/plasma kit for my serum samples. After the phase separation the samples should have 3 phases: a colourless aqueous phase, a white interphase and a red organic phase. However, in some of my samples there was no aqueous phase unless I wait for an extended period of time. How can I circumvent this problem?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Tissue - Human Joints using RNeasy Fibrous Tissue Mini Kit from Qiagen.

Paper title
Functional Tissue Analysis Reveals Successful Cryopreservation of Human Osteoarthritic Synovium
Full paper
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Manufacturer protocol

Download the product protocol from Qiagen for RNeasy Fibrous Tissue Mini Kit below.

Download PDF Download manufacturer protocol

Videos

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