TRI Reagent® Sigma

RNA isolation / purification Tissue - Human Joints

Experiment
RNA isolation / purification Tissue - Human Joints
Product
TRI Reagent® Sigma from Sigma-Aldrich
Manufacturer
Sigma-Aldrich

Protocol tips

Upstream tips
- 1-Bromo-3-chloropropane is less toxic than chloroform and its use for phase separation decreases the possibility of contaminating RNA with DNA

- The chloroform used for phase separation should not contain isoamyl alcohol or other additives.

Publication protocol

Tissue samples from every single patient were frozen in liquid nitrogen immediately after dissection and stored at -70°C until homogenization. Sample preparation for gene array analysis was carried out as previously described [25–27]. Briefly, frozen samples were homogenized in TRI REAGENT (1mL per 50–100mg of tissue) in a Potter-type mechanical homogenizer with Teflon pestle.

PAXgene Blood RNA tubes (PreAnalytiX, Hombrechtikon, Switzerland) were used for blood collection and total RNA was extracted according to the protocol supplied by the manufacturer.

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Discussion

Discussion

4 years ago

Author: Paul G. Macon United States

RNA isolation from tissue

How do I extract RNA from animal tissue without using liquid nitrogen? I tried the RNA extraction by using the TRIzol reagent and I homogenize the tissue using polytron homogenizer at room temperature for 30secs is this correct?

Discussion

4 years ago

Author: Aaron Stege Netherlands

Problem in phase separation after using serum/plasma kit

I used a serum/plasma kit for my serum samples. After the phase separation the samples should have 3 phases: a colourless aqueous phase, a white interphase and a red organic phase. However, in some of my samples there was no aqueous phase unless I wait for an extended period of time. How can I circumvent this problem?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Tissue - Human Joints using TRI Reagent® Sigma from Sigma-Aldrich.

Paper title
Gene Expression Profiling in Peripheral Blood Cells and Synovial Membranes of Patients with Psoriatic Arthritis
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Manufacturer protocol

Download the product protocol from Sigma-Aldrich for TRI Reagent® Sigma below.

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