mirVana™ miRNA Isolation Kit, with phenol

RNA isolation / purification Tissue - Human Mouth

Experiment
RNA isolation / purification Tissue - Human Mouth
Product
mirVana™ miRNA Isolation Kit, with phenol from Thermo Fisher Scientific
Manufacturer
Thermo Fisher Scientific

Protocol tips

Upstream tips

Protocol tips
- For RNA to dissolve better and to yeild high levels, preheat elution buffer at 95C.

- Addition of β-mercaptoethanol in lysis buffer can be skipped because the phenol will do the job of nuclease inactivation

- To capture more amount of mRNA, modify the amount of lysis buffer :ethanol (from 1:1 to 1:1.5) during the binding step
Downstream tips
- Do vortex for 2-plus minutes during Acid-Phenol:Chloroform extraction step. This will facilitate the removal of tightly bound proteins typically associated with RNA

Publication protocol

Gingival tissue was homogenized thoroughly using a tissue homogenizer, and then total cellular RNA was isolated with a total RNA isolation kit according to the manufacturer's instructions (mirVANA miRNA isolation kit, Ambion by Life Technologies, India). The total RNA obtained was treated with RNase-free DNase to remove any contaminating DNA. The eluate (which contains the RNA) was collected and stored at −20°C or below

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Discussion

Discussion

1 year ago

Author: Paul G. Macon United States

RNA isolation from tissue

How do I extract RNA from animal tissue without using liquid nitrogen? I tried the RNA extraction by using the TRIzol reagent and I homogenize the tissue using polytron homogenizer at room temperature for 30secs is this correct?

Discussion

2 years ago

Author: Aaron Stege Netherlands

Problem in phase separation after using serum/plasma kit

I used a serum/plasma kit for my serum samples. After the phase separation the samples should have 3 phases: a colourless aqueous phase, a white interphase and a red organic phase. However, in some of my samples there was no aqueous phase unless I wait for an extended period of time. How can I circumvent this problem?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Tissue - Human Mouth using mirVana™ miRNA Isolation Kit, with phenol from Thermo Fisher Scientific.

Paper title
mRNA Expression of MMP-28 (Epilysin) in Gingival Tissues of Chronic and Aggressive Periodontitis Patients: A Reverse Transcriptase PCR Study
Full paper
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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for mirVana™ miRNA Isolation Kit, with phenol below.

Download PDF Download manufacturer protocol

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