MEDOX-Easy Total RNA Extraction Reagent

RNA isolation / purification Tissue - Human Mouth

Experiment
RNA isolation / purification Tissue - Human Mouth
Product
MEDOX-Easy Total RNA Extraction Reagent from Medox Biotech
Manufacturer
Medox Biotech

Protocol tips

Upstream tips
- This kit performs well with small quantities of tissue (50~100mg)

Publication protocol

The tissue samples were immersed in an RNAase inhibitor containing solution (RNA later, Invitrogen, Bangalore, India) and homogenized immediately. Total RNA was obtained from the homogenates using Trizol total RNA extraction kit (Medox, Chennai, Tamil Nadu, India). The first step in real time reverse transcription polymerase chain reaction (q-PCR) was the conversion of the total RNA to a complementary cDNA which was then carried out using a high capacity cDNA reverse transcription kit (Applied Biosystems, Invitrogen BioServices, Bangalore, India). The quantitative real-time amplification was done using a SYBR Green containing master mix kit (Qiagen, Germany) to evaluate the mRNA expression of T-bet and GATA-3 in periodontal health and disease.

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Discussion

Discussion

1 year ago

Author: Paul G. Macon United States

RNA isolation from tissue

How do I extract RNA from animal tissue without using liquid nitrogen? I tried the RNA extraction by using the TRIzol reagent and I homogenize the tissue using polytron homogenizer at room temperature for 30secs is this correct?

Discussion

2 years ago

Author: Aaron Stege Netherlands

Problem in phase separation after using serum/plasma kit

I used a serum/plasma kit for my serum samples. After the phase separation the samples should have 3 phases: a colourless aqueous phase, a white interphase and a red organic phase. However, in some of my samples there was no aqueous phase unless I wait for an extended period of time. How can I circumvent this problem?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Tissue - Human Mouth using MEDOX-Easy Total RNA Extraction Reagent from Medox Biotech.

Paper title
Evaluation of mRNA expression of the transcription factors of Th1 and Th2 subsets (T-bet and GATA-3) in periodontal health and disease - A pilot study in south Indian population
Full paper
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Manufacturer protocol

Download the product protocol from Medox Biotech for MEDOX-Easy Total RNA Extraction Reagent below.

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