miRNeasy Mini kit

RNA isolation / purification Tissue - Human Pituitary gland

Experiment
RNA isolation / purification Tissue - Human Pituitary gland
Product
miRNeasy Mini kit from Qiagen
Manufacturer
Qiagen

Protocol tips

Protocol tips
To capture a higher amount of mRNA, modify the amount of lysis buffer :ethanol (from 1:1 to 1:1.5) during the binding step.

Publication protocol

RNA was isolated using the miRNeasy Mini Kit (Qiagen) and the Qiagen tissue lyser using 7 mm stainless steel beads. Tissue samples were disrupted for 5 min 30 Hz (1800 oscillations/min) in Qiazol lysis reagent. Further purification was done according to manufacturer's instructions. Concentration and purity was measured using NanoDrop 2000 (Thermo Scientific). RNA integrity was measured using Bioanalyzer RNA Nano Chip (Agilent). As expected for autopsy samples, the RNA integrity values (RIN) ranged between 1.8 and 2.7.

Full paper   Login or join for free to view the full paper.

Reviews

miRNeasy Mini kit from Qiagen has not yet been reviewed for this experiment

We'd love it if you would be the first to write a review!

Discussion

Discussion

1 year ago

Author: Paul G. Macon United States

RNA isolation from tissue

How do I extract RNA from animal tissue without using liquid nitrogen? I tried the RNA extraction by using the TRIzol reagent and I homogenize the tissue using polytron homogenizer at room temperature for 30secs is this correct?

Discussion

1 year ago

Author: Aaron Stege Netherlands

Problem in phase separation after using serum/plasma kit

I used a serum/plasma kit for my serum samples. After the phase separation the samples should have 3 phases: a colourless aqueous phase, a white interphase and a red organic phase. However, in some of my samples there was no aqueous phase unless I wait for an extended period of time. How can I circumvent this problem?

Share your thoughts or question with experts in your field by adding a discussion!

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Tissue - Human Pituitary gland using miRNeasy Mini kit from Qiagen.

View full paper   Login or join for free to view the full paper.

Manufacturer protocol

Download the product protocol from Qiagen for miRNeasy Mini kit below.

Download PDF Download manufacturer protocol

Videos

Check out videos that might be relevant for performing RNA isolation / purification Tissue - Human Pituitary gland using miRNeasy Mini kit from Qiagen. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.

We haven't found any additional videos for this experiment / product combination yet.

Become shareholder Discussions About us Contact Privacy Terms