MagNA Pure Compact RNA Isolation Kit
RNA isolation / purification Tissue - Human Placenta
Publication protocol
Disruption and homogenization of tissue specimens were performed in lysis buffer using the MagNa Lyser Instrument (Roche Applied Science, Germany), according to the manufacturer's instructions. Isolation of total RNA was performed using the MagNa Pure Compact RNA isolation kit and the MagNa Pure Compact Instrument (Roche Applied Science, Germany). RNA was quantified by measuring absorbance at 260 nm, and RNA purity was determined by the ratios OD260 nm/280 nm and OD230 nm/280 nm using the NanoDrop instrument (NanoDrop® ND-1000, Wilmington, USA). The RNA integrity was determined by electrophoresis using the Agilent 2100 Bioanalyser (Matriks, Norway). Only samples with RNA Integrity Number (RIN) >7.2 were used for microarray.Full paper Login or join for free to view the full paper.
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Discussion
Discussion
4 years ago
Author:
Paul G. Macon
RNA isolation from tissue
How do I extract RNA from animal tissue without using liquid nitrogen? I tried the RNA extraction by using the TRIzol reagent and I homogenize the tissue using polytron homogenizer at room temperature for 30secs is this correct?
Discussion
4 years ago
Author:
Aaron Stege
Problem in phase separation after using serum/plasma kit
I used a serum/plasma kit for my serum samples. After the phase separation the samples should have 3 phases: a colourless aqueous phase, a white interphase and a red organic phase. However, in some of my samples there was no aqueous phase unless I wait for an extended period of time. How can I circumvent this problem?
Papers
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Manufacturer protocol
Download the product protocol from Roche Lifesciences for MagNA Pure Compact RNA Isolation Kit below.
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