Aurum™ Total RNA Mini Kit

RNA isolation / purification Tissue - Human Subcutaneous

Experiment
RNA isolation / purification Tissue - Human Subcutaneous
Product
Aurum™ Total RNA Mini Kit from Bio-Rad Laboratories
Manufacturer
Bio-Rad Laboratories

Protocol tips

Upstream tips
- Before using lysis solution, add 500 ul of β-mercaptoethanol (β-ME) to the solution for a final concentration of 1%.
Protocol tips
- To perform efficient elution preheat the elution solution at 70C in water bath priorto the elution step.

- If there is high genomic DNA contamination, increase DNAse I digestion time and use only the DNAse dilution solution provided in the kit
Downstream tips
- For better downstream application add appropriate volume of 95-100% ethanol to the wash solutions before intial use.

- If elute volume is >80ul, there is a possibility of ethanol contamination. To reduce this, add 1-3min of centrifugation time after the final wash step.

Publication protocol

RNA was isolated (Aurum Total RNA Mini kit, Bio Rad) and stored at −80 °C until use. RNA labeling and hybridization on microRNA microarray chips was performed

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Reviews

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Discussion

Discussion

1 year ago

Author: Paul G. Macon United States

RNA isolation from tissue

How do I extract RNA from animal tissue without using liquid nitrogen? I tried the RNA extraction by using the TRIzol reagent and I homogenize the tissue using polytron homogenizer at room temperature for 30secs is this correct?

Discussion

2 years ago

Author: Aaron Stege Netherlands

Problem in phase separation after using serum/plasma kit

I used a serum/plasma kit for my serum samples. After the phase separation the samples should have 3 phases: a colourless aqueous phase, a white interphase and a red organic phase. However, in some of my samples there was no aqueous phase unless I wait for an extended period of time. How can I circumvent this problem?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Tissue - Human Subcutaneous using Aurum™ Total RNA Mini Kit from Bio-Rad Laboratories.

Paper title
Gene expression regional differences in human subcutaneous adipose tissue
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Manufacturer protocol

Download the product protocol from Bio-Rad Laboratories for Aurum™ Total RNA Mini Kit below.

Download PDF Download manufacturer protocol

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