TRIzol Reagent
RNA isolation / purification Tissue - Human Tongue
Protocol tips
| Protocol tips |
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| - For low 260/230 readings the best approach is to try washing sample (precipitation) with ethanol to desalt it. |
Publication protocol
Tongue and cheek saliva samples were collected using an in-house developed method (unpublished data). Participants were instructed to gently scrape their teeth back and forth across the front of the tongue or gently scrape the teeth over the cheeks and expectorate the saliva into a 15ml centrifuge tube (total 2ml). Centrifuge tubes were kept on ice during the collection procedure (2-10min) and were snap frozen in dry ice and stored at -80°C. To extract the RNA, samples were thawed on ice and 2ml of PBS added and mixed well to dilute the salivary mucins. The samples were then centrifuged (850rcf, 5min), the supernatant discarded and 1ml of TRIzol added to the samples.Full paper Login or join for free to view the full paper.
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Discussion
Discussion
4 years ago
Author:
Paul G. Macon
RNA isolation from tissue
How do I extract RNA from animal tissue without using liquid nitrogen? I tried the RNA extraction by using the TRIzol reagent and I homogenize the tissue using polytron homogenizer at room temperature for 30secs is this correct?
Discussion
4 years ago
Author:
Aaron Stege
Problem in phase separation after using serum/plasma kit
I used a serum/plasma kit for my serum samples. After the phase separation the samples should have 3 phases: a colourless aqueous phase, a white interphase and a red organic phase. However, in some of my samples there was no aqueous phase unless I wait for an extended period of time. How can I circumvent this problem?
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Manufacturer protocol
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