RNeasy Lipid Tissue Mini Kit

RNA isolation / purification Tissue - Mouse Adipose

Experiment
RNA isolation / purification Tissue - Mouse Adipose
Product
RNeasy Lipid Tissue Mini Kit from Qiagen
Manufacturer
Qiagen

Protocol tips

Protocol tips
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- If the RNA yield is low, repeat RNA elution, but incubate the RNeasy Mini spin column on the benchtop for 10 min with RNase-free water before centrifuging.

- All centrifugation steps should be performed at 15–25°C except for phase separation

Publication protocol

Perfused epididymal adipose tissues harvested from LPS-injected mice were cut into 30 mg (approximate size) pieces and digested with 1 mg/mL collagenase (Type I, Sigma-Aldrich, St. Louis, MO, USA) dissolved in 2% BSA-HBSS + 5mM glucose for 1h with agitation at 37°C. Digested cells were then passed through a 100μm cell strainer and the strainer was washed with HBSS, followed by centrifugation for 10 minutes. Floating adipocytes and buffer were transferred to a new tube, followed by a second centrifugation. The pellet containing the SVF was resuspended in HBSS and transferred to a new tube, followed by centrifugation. All centrifugation steps were performed at 4°C and 450 × g. Total RNA was isolated using RNeasy® Lipid Tissue Mini Kit (Qiagen, Valenica, CA, USA).

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Reviews

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Discussion

Discussion

2 years ago

Author: Paul G. Macon United States

RNA isolation from tissue

How do I extract RNA from animal tissue without using liquid nitrogen? I tried the RNA extraction by using the TRIzol reagent and I homogenize the tissue using polytron homogenizer at room temperature for 30secs is this correct?

Discussion

3 years ago

Author: Aaron Stege Netherlands

Problem in phase separation after using serum/plasma kit

I used a serum/plasma kit for my serum samples. After the phase separation the samples should have 3 phases: a colourless aqueous phase, a white interphase and a red organic phase. However, in some of my samples there was no aqueous phase unless I wait for an extended period of time. How can I circumvent this problem?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Tissue - Mouse Adipose using RNeasy Lipid Tissue Mini Kit from Qiagen.

Paper title
GENE EXPRESSION PROFILE OF MOUSE WHITE ADIPOSE TISSUE DURING INFLAMMATORY STRESS: AGE-DEPENDENT UPREGULATION OF MAJOR PRO-COAGULANT FACTORS
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Manufacturer protocol

Download the product protocol from Qiagen for RNeasy Lipid Tissue Mini Kit below.

Download PDF Download manufacturer protocol

Videos

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