SV Total RNA Isolation System

RNA isolation / purification Tissue - Mouse Adrenal glands

Experiment
RNA isolation / purification Tissue - Mouse Adrenal glands
Product
SV Total RNA Isolation System from Promega
Manufacturer
Promega

Protocol tips

Protocol tips
- Do not freeze-thaw the DNase more than three times after rehydration

Publication protocol

RNA extraction was performed using the SV Total RNA Isolation System according to the manufacturer's instructions (Promega). RNA quantification was performed with a NanoDrop spectrophotometer. The 260/280 absorbance ratio was used to asses RNA purity (mean ratio, 2025 ± 0.01). In addition, RNA integrity was assessed by running an aliquot on denaturing agarose gel stained with ethidium bromide. For cDNA synthesis, 500 ng of total RNA was reverse transcribed using a reverse transcription system (Promega).

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Discussion

Discussion

4 years ago

Author: Paul G. Macon United States

RNA isolation from tissue

How do I extract RNA from animal tissue without using liquid nitrogen? I tried the RNA extraction by using the TRIzol reagent and I homogenize the tissue using polytron homogenizer at room temperature for 30secs is this correct?

Discussion

4 years ago

Author: Aaron Stege Netherlands

Problem in phase separation after using serum/plasma kit

I used a serum/plasma kit for my serum samples. After the phase separation the samples should have 3 phases: a colourless aqueous phase, a white interphase and a red organic phase. However, in some of my samples there was no aqueous phase unless I wait for an extended period of time. How can I circumvent this problem?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Tissue - Mouse Adrenal glands using SV Total RNA Isolation System from Promega.

Paper title
Adrenal and Ovarian Phenotype of a Tissue-Specific Urocortin 2-Overexpressing Mouse Model.
Full paper
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Manufacturer protocol

Download the product protocol from Promega for SV Total RNA Isolation System below.

Download PDF Download manufacturer protocol

Videos

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