RNeasy Mini Kit

RNA isolation / purification Tissue - Mouse Artery / aorta

Experiment

RNA isolation / purification Tissue - Mouse Artery / aorta

Product

RNeasy Mini Kit from Qiagen

Manufacturer

Qiagen

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Tips Publication protocol Reviews Discussion Papers Manufacturer protocol Videos

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	- To yield high RNA, give the column an extra wash with both RW1 and RPE buffers (3 total washes with each buffer).	- Include DNAse treatment for 15-20min. - Ensure EtOH is completely evaporated off of the column prior to elution. Adjust time from 1min to 5 min at 60`C. - Use water to elute the RNA that is warmed to ~60`C.

Protocol tips
- To yield high RNA, give the column an extra wash with both RW1 and RPE buffers (3 total washes with each buffer).
Downstream tips
- Include DNAse treatment for 15-20min. - Ensure EtOH is completely evaporated off of the column prior to elution. Adjust time from 1min to 5 min at 60`C. - Use water to elute the RNA that is warmed to ~60`C.

Publication protocol

Serum TNC levels were determined with an ELISA kit (Immuno-Biological Laboratories). Serum cytokine levels were determined with the Bio-Plex system (Bio-Rad). We isolated total RNA using RNeasy (Qiagen) from SMC culture or suprarenal aorta (from the edge of the right renal artery to approximately 7 mm above) cleaned of peri-adventitial loose connective tissue with intact medial and adventitial layers. We performed transcriptome analyses using the GeneChip Mouse Genome 430 2.0 (Affymetrix) and Microarray Analysis Suite (MAS) 5.0. We also performed quantitative reverse transcription-polymerase chain reaction (RT-PCR) using RT2 Profiler PCR Array System (Qiagen

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Discussion

4 years ago

Author: Paul G. Macon United States

RNA isolation from tissue

How do I extract RNA from animal tissue without using liquid nitrogen? I tried the RNA extraction by using the TRIzol reagent and I homogenize the tissue using polytron homogenizer at room temperature for 30secs is this correct?

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Discussion

4 years ago

Author: Aaron Stege Netherlands

Problem in phase separation after using serum/plasma kit

I used a serum/plasma kit for my serum samples. After the phase separation the samples should have 3 phases: a colourless aqueous phase, a white interphase and a red organic phase. However, in some of my samples there was no aqueous phase unless I wait for an extended period of time. How can I circumvent this problem?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Tissue - Mouse Artery / aorta using RNeasy Mini Kit from Qiagen.

Paper title

Tenascin C protects aorta from acute dissection in mice

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Manufacturer protocol

Download the product protocol from Qiagen for RNeasy Mini Kit below.

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Videos

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