NucleoSpin® RNA

RNA isolation / purification Tissue - Mouse Cerebellum

Experiment
RNA isolation / purification Tissue - Mouse Cerebellum
Product
NucleoSpin® RNA from Macherey Nagel
Manufacturer
Macherey Nagel

Protocol tips

Upstream tips
- Aliquot rDNase and store at -20 °C.
Protocol tips
- Dry the columns very well after the ethanol wash by adding an additional 2' >10000 rpm centrifuge with no buffer.

Publication protocol

RNA was isolated from the cerebellum of WT [A], A-T [A] and A-T [M] mice using a Nucleospin RNA kit (Macherey-Nagel 740955) as per manufacturer’s protocol.

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Discussion

Discussion

2 years ago

Author: Paul G. Macon United States

RNA isolation from tissue

How do I extract RNA from animal tissue without using liquid nitrogen? I tried the RNA extraction by using the TRIzol reagent and I homogenize the tissue using polytron homogenizer at room temperature for 30secs is this correct?

Discussion

2 years ago

Author: Aaron Stege Netherlands

Problem in phase separation after using serum/plasma kit

I used a serum/plasma kit for my serum samples. After the phase separation the samples should have 3 phases: a colourless aqueous phase, a white interphase and a red organic phase. However, in some of my samples there was no aqueous phase unless I wait for an extended period of time. How can I circumvent this problem?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Tissue - Mouse Cerebellum using NucleoSpin® RNA from Macherey Nagel.

Paper title
A novel mouse model for ataxia-telangiectasia with a N-terminal mutation displays a behavioral defect and a low incidence of lymphoma but no increased oxidative burden
Full paper
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Manufacturer protocol

Download the product protocol from Macherey Nagel for NucleoSpin® RNA below.

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Videos

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