illustra RNAspin Mini Kit

RNA isolation / purification Tissue - Mouse Jejunum

Experiment
RNA isolation / purification Tissue - Mouse Jejunum
Product
illustra RNAspin Mini Kit from GE Healthcare Life Sciences
Manufacturer
GE Healthcare Life Sciences

Protocol tips

Upstream tips
- This kit can be used for the isolation of RNA from up to 5x10^6 cells

Publication protocol

The jejunum was aseptically removed from mice killed by cervical dislocation 24 h after oral challenge and mRNA was isolated using illustra RNAspin mini isolation kit according the manufacturer’s specifications (GE Healthcare)

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Reviews

illustra RNAspin Mini Kit from GE Healthcare Life Sciences has not yet been reviewed for this experiment

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Discussion

Discussion

2 years ago

Author: Paul G. Macon United States

RNA isolation from tissue

How do I extract RNA from animal tissue without using liquid nitrogen? I tried the RNA extraction by using the TRIzol reagent and I homogenize the tissue using polytron homogenizer at room temperature for 30secs is this correct?

Discussion

3 years ago

Author: Aaron Stege Netherlands

Problem in phase separation after using serum/plasma kit

I used a serum/plasma kit for my serum samples. After the phase separation the samples should have 3 phases: a colourless aqueous phase, a white interphase and a red organic phase. However, in some of my samples there was no aqueous phase unless I wait for an extended period of time. How can I circumvent this problem?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Tissue - Mouse Jejunum using illustra RNAspin Mini Kit from GE Healthcare Life Sciences.

Paper title
Oral delivery of spp. recombinant protein U-Omp16 abrogates the IgE-mediated milk allergy
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Manufacturer protocol

Download the product protocol from GE Healthcare Life Sciences for illustra RNAspin Mini Kit below.

Download PDF Download manufacturer protocol

Videos

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