High Pure RNA Isolation Kit

RNA isolation / purification Tissue - Mouse Kidney

Experiment
RNA isolation / purification Tissue - Mouse Kidney
Product
High Pure RNA Isolation Kit from Roche Lifesciences
Manufacturer
Roche Lifesciences

Protocol tips

Upstream tips
- Be careful to create an RNase-free working environment
Protocol tips
- Always mix the sample tube well after addition of each reagent.

Publication protocol

Tissues from adult male mice were collected: brain, lung, liver, spleen, kidney, heart, and testis (1-week to 8-month old); then homogenized and total RNA was extracted using High Pure RNA Isolation Kit (Roche, Indianapolis, IN). Total RNA concentration was validated and measured using a Nanodrop Spectrophotometer (BioDrop, Cambridge, UK). cDNA was synthesized from RNA samples using iScriptTM cDNA Synthesis Kit (Bio-Rad, Hercules, CA) in 20 μL reaction volume. Starting amount of total RNA used was 500 ng.

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Reviews

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Discussion

Discussion

2 years ago

Author: Paul G. Macon United States

RNA isolation from tissue

How do I extract RNA from animal tissue without using liquid nitrogen? I tried the RNA extraction by using the TRIzol reagent and I homogenize the tissue using polytron homogenizer at room temperature for 30secs is this correct?

Discussion

3 years ago

Author: Aaron Stege Netherlands

Problem in phase separation after using serum/plasma kit

I used a serum/plasma kit for my serum samples. After the phase separation the samples should have 3 phases: a colourless aqueous phase, a white interphase and a red organic phase. However, in some of my samples there was no aqueous phase unless I wait for an extended period of time. How can I circumvent this problem?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Tissue - Mouse Kidney using High Pure RNA Isolation Kit from Roche Lifesciences.

Paper title
Changes in Protein O-GlcNAcylation During Mouse Epididymal Sperm Maturation
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Manufacturer protocol

Download the product protocol from Roche Lifesciences for High Pure RNA Isolation Kit below.

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Videos

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