PicoPure™ RNA Isolation Kit

RNA isolation / purification Tissue - Mouse Larynx

Experiment
RNA isolation / purification Tissue - Mouse Larynx
Product
PicoPure™ RNA Isolation Kit from Thermo Fisher Scientific
Manufacturer
Thermo Fisher Scientific

Protocol tips

Upstream tips
- Can be used when there is a very less amount of starting material

Publication protocol

ibonucleic acid was isolated from the lamina propria using Picopure RNA isolation kit (Arcturus). Quality check for RNA was determined using ABI taqman assay (RPL13a assay); the Ct value cut off was 30. Total RNA of 250ng was processed for Human whole genome Illumina DASL kit for micro array analysis.

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Reviews

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Discussion

Discussion

2 years ago

Author: Paul G. Macon United States

RNA isolation from tissue

How do I extract RNA from animal tissue without using liquid nitrogen? I tried the RNA extraction by using the TRIzol reagent and I homogenize the tissue using polytron homogenizer at room temperature for 30secs is this correct?

Discussion

3 years ago

Author: Aaron Stege Netherlands

Problem in phase separation after using serum/plasma kit

I used a serum/plasma kit for my serum samples. After the phase separation the samples should have 3 phases: a colourless aqueous phase, a white interphase and a red organic phase. However, in some of my samples there was no aqueous phase unless I wait for an extended period of time. How can I circumvent this problem?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Tissue - Mouse Larynx using PicoPure™ RNA Isolation Kit from Thermo Fisher Scientific.

Paper title
Radiation Fibrosis of the Vocal Fold: From Man to Mouse
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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for PicoPure™ RNA Isolation Kit below.

Download PDF Download manufacturer protocol

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