Total RNA Purification Kit

RNA isolation / purification Tissue - Mouse Lung

Experiment
RNA isolation / purification Tissue - Mouse Lung
Product
Total RNA Purification Kit from Norgen Biotek
Manufacturer
Norgen Biotek

Protocol tips

Upstream tips
- For lysis/cell disruption check the protocol beforehand to see which reagents/lab materials are needed
Downstream tips
- Minimal DNA is left over using this kit, however if DNA is undesired an additional RNase free DNase treatment is warranted.

Publication protocol

Total RNA was isolated using the following kits: Isolate II RNA mini kit (Bioline, London, UK), miRVana microRNA isolation kit (Thermo Fisher Scientific, Waltham, MA, USA), Total RNA isolation kit (Norgen Biotek, Thorold, ON, Canada), miRNeasy mini kit (Qiagen, Hilden, Germany), and TRIzol Reagent (Thermo Fisher Scientific)

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Reviews

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Discussion

Discussion

2 years ago

Author: Paul G. Macon United States

RNA isolation from tissue

How do I extract RNA from animal tissue without using liquid nitrogen? I tried the RNA extraction by using the TRIzol reagent and I homogenize the tissue using polytron homogenizer at room temperature for 30secs is this correct?

Discussion

3 years ago

Author: Aaron Stege Netherlands

Problem in phase separation after using serum/plasma kit

I used a serum/plasma kit for my serum samples. After the phase separation the samples should have 3 phases: a colourless aqueous phase, a white interphase and a red organic phase. However, in some of my samples there was no aqueous phase unless I wait for an extended period of time. How can I circumvent this problem?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Tissue - Mouse Lung using Total RNA Purification Kit from Norgen Biotek.

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Manufacturer protocol

Download the product protocol from Norgen Biotek for Total RNA Purification Kit below.

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Videos

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