|- To yield high RNA, give the column an extra wash with both RW1 and RPE buffers (3 total washes with each buffer).|
|- Include DNAse treatment for 15-20min.
- Ensure EtOH is completely evaporated off of the column prior to elution. Adjust time from 1min to 5 min at 60`C.
- Use water to elute the RNA that is warmed to ~60`C.
RNeasy Mini Kit from Qiagen has not yet been reviewed for this experiment
We'd love it if you would be the first to write a review!
2 years ago
Author: Paul G. Macon
How do I extract RNA from animal tissue without using liquid nitrogen? I tried the RNA extraction by using the TRIzol reagent and I homogenize the tissue using polytron homogenizer at room temperature for 30secs is this correct?
3 years ago
Author: Aaron Stege
I used a serum/plasma kit for my serum samples. After the phase separation the samples should have 3 phases: a colourless aqueous phase, a white interphase and a red organic phase. However, in some of my samples there was no aqueous phase unless I wait for an extended period of time. How can I circumvent this problem?
Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Tissue - Mouse Lymph node using RNeasy Mini Kit from Qiagen.
Download the product protocol from Qiagen for RNeasy Mini Kit below.Download manufacturer protocol
Check out videos that might be relevant for performing RNA isolation / purification Tissue - Mouse Lymph node using RNeasy Mini Kit from Qiagen. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.
We haven't found any additional videos for this experiment / product combination yet.
Fill out your contact details and receive price quotes in your InboxOutsource experiment