Lipofectamine® LTX Reagent

DNA transfection Mammalian cells - Immortalized cell lines BXPC3

Experiment
DNA transfection Mammalian cells - Immortalized cell lines BXPC3
Product
Lipofectamine® LTX Reagent from Thermo Fisher Scientific
Manufacturer
Thermo Fisher Scientific

Protocol tips

Protocol tips
Do not add antibiotics to the Transfection Medium

Publication protocol

Transfection of plasmid DNA
Transient transfection of human Bip promoter reporter plasmid containing firefly luciferase (2 µg of pBipPro‐Luc), in which the Bip promoter region was cloned into the pGL4.14 vector (Promega, Madison, WI, USA) as described previously 10, was performed using Lipofectamine LTX (Invitrogen, Carlsbad, CA, USA) according to the manufacturer's protocol 11. The reporter assay using a luminometer was performed using 0.2 µg of pRL‐SV40 plasmid DNA (Promega) as an internal control by the Dual‐Glo Luciferase Assay System (Promega) as described previously 11. The same concentration of plasmid DNAs was used in all cell lines. Thapsigargin (Tg) was used for the induction of endoplasmic reticulum (ER) stress after transfection as described previously 11-13.

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Papers

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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for Lipofectamine® LTX Reagent below.

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