PicoPure™ RNA Isolation Kit

RNA isolation / purification Tissue - Mouse Pancreas

Experiment
RNA isolation / purification Tissue - Mouse Pancreas
Product
PicoPure™ RNA Isolation Kit from Thermo Fisher Scientific
Manufacturer
Thermo Fisher Scientific

Protocol tips

Upstream tips
- Can be used when there is a very less amount of starting material

Publication protocol

Pancreata were removed from 15-week-old mice, and their sections were adhered to non-coated glass slides, fixed by treatment with 75% ethanol for 30 s at −18 °C, and then dehydrated. The PixCell IIe Laser Capture Microdissection System (Arcturus, Mountain View, CA, USA) was used to isolate 50–70 islet pieces that were laser captured to CapSur (Arcturus). Cell homogeneity was confirmed microscopically prior to processing for RNA extraction. Total RNA was subsequently extracted using the Pico Pure RNA Isolation kit (Arcturus) according to the manufacturer's protocols.

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Discussion

Discussion

2 years ago

Author: Paul G. Macon United States

RNA isolation from tissue

How do I extract RNA from animal tissue without using liquid nitrogen? I tried the RNA extraction by using the TRIzol reagent and I homogenize the tissue using polytron homogenizer at room temperature for 30secs is this correct?

Discussion

3 years ago

Author: Aaron Stege Netherlands

Problem in phase separation after using serum/plasma kit

I used a serum/plasma kit for my serum samples. After the phase separation the samples should have 3 phases: a colourless aqueous phase, a white interphase and a red organic phase. However, in some of my samples there was no aqueous phase unless I wait for an extended period of time. How can I circumvent this problem?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Tissue - Mouse Pancreas using PicoPure™ RNA Isolation Kit from Thermo Fisher Scientific.

Paper title
Comparative gene expression profiles in pancreatic islets associated with agouti yellow mutation and PACAP overexpression in mice
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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for PicoPure™ RNA Isolation Kit below.

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