AllPrep DNA/RNA FFPE Kit

RNA isolation / purification Tissue - Mouse Prostate

Experiment
RNA isolation / purification Tissue - Mouse Prostate
Product
AllPrep DNA/RNA FFPE Kit from Qiagen
Manufacturer
Qiagen

Protocol tips

Upstream tips
- To allow complete penetration by formalin, use tissue samples less than 5 mm thick. If buffers are precipitated dissolve them by heating to 70°C with gentle agitation
Protocol tips
- Perform all centrifugation steps using a microcentrifuge placed at 15–25°C
Downstream tips
- Do not exceed fixation time of 24 hours as it results in poor performance in downstream assay

Publication protocol

The kit deemed most suitable for FFPE tissue was the AllPrep kit by Qiagen because of its yield, quality, and ability to purify both RNA and DNA from the same sample, which would be advantageous in biomarker studies.

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Reviews

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Discussion

Discussion

4 years ago

Author: Paul G. Macon United States

RNA isolation from tissue

How do I extract RNA from animal tissue without using liquid nitrogen? I tried the RNA extraction by using the TRIzol reagent and I homogenize the tissue using polytron homogenizer at room temperature for 30secs is this correct?

Discussion

4 years ago

Author: Aaron Stege Netherlands

Problem in phase separation after using serum/plasma kit

I used a serum/plasma kit for my serum samples. After the phase separation the samples should have 3 phases: a colourless aqueous phase, a white interphase and a red organic phase. However, in some of my samples there was no aqueous phase unless I wait for an extended period of time. How can I circumvent this problem?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Tissue - Mouse Prostate using AllPrep DNA/RNA FFPE Kit from Qiagen.

Paper title
Reliability and performance of commercial RNA and DNA extraction kits for FFPE tissue cores
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Manufacturer protocol

Download the product protocol from Qiagen for AllPrep DNA/RNA FFPE Kit below.

Download PDF Download manufacturer protocol

Videos

Check out videos that might be relevant for performing RNA isolation / purification Tissue - Mouse Prostate using AllPrep DNA/RNA FFPE Kit from Qiagen. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.

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