NucleoSpin® RNA
RNA isolation / purification Tissue - Mouse Prostate
Protocol tips
| Protocol tips |
|---|
| - Aliquot rDNase and store at -20 °C. |
| Downstream tips |
| Dry the columns very well after the ethanol wash by adding an additional 2' >10000 rpm centrifuge with no buffer. |
Publication protocol
Total RNAs were isolated using NucleoSpin RNA II column kit (Macherey-Nagel, Hoerd, France). cDNAs were synthesized with Moloney Murine Leukemia Virus Reverse Transcriptase (Promega) and random hexamer primers (Promega) according to the manufacturer's instructions. cDNA templates were amplified by MESA GREEN MasterMix Plus for SYBR Assay (Eurogentec, Seraing, Belgium) using an iCycler (Bio-Rad, Marnes-la-Coquette, France). Primer sequences are listed in Text S1. qPCR results were normalized alternatively using 36b4 or 18S as a standard.Full paper Login or join for free to view the full paper.
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Discussion
Discussion
4 years ago
Author:
Paul G. Macon
RNA isolation from tissue
How do I extract RNA from animal tissue without using liquid nitrogen? I tried the RNA extraction by using the TRIzol reagent and I homogenize the tissue using polytron homogenizer at room temperature for 30secs is this correct?
Discussion
4 years ago
Author:
Aaron Stege
Problem in phase separation after using serum/plasma kit
I used a serum/plasma kit for my serum samples. After the phase separation the samples should have 3 phases: a colourless aqueous phase, a white interphase and a red organic phase. However, in some of my samples there was no aqueous phase unless I wait for an extended period of time. How can I circumvent this problem?
Papers
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Manufacturer protocol
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