PureLink™ RNA Mini Kit

RNA isolation / purification Tissue - Mouse Spleen

Experiment
RNA isolation / purification Tissue - Mouse Spleen
Product
PureLink™ RNA Mini Kit from Thermo Fisher Scientific
Manufacturer
Thermo Fisher Scientific

Protocol tips

Protocol tips
- Depending on the material you want to isolate RNA from, please refer to the manual for additional reagents/lab equipment that are required
Downstream tips
- DNase is not supplied with this kit, a DNase kit is available which is compatible with this kit

Publication protocol

Ten milligrams of tissue mass was used to obtain high quality, DNA-free, RNA with Pure Link RNA Mini Kit (Ambion, Carlsbad, CA, U.S.A.) according to manufacturer’s instructions, to ensure high-quality DNAse solution was applied to each sample and traces of DNAse were eliminated by additional washing steps. NanoDrop 1000 spectrophotometer (PEQLAB Biotechnologie, Erlangen, Germany) was used to assess the concentration and purity of aqueous RNA samples as described [48,49]. In brief, the absorbance ratio 260/280 was calculated and values between 1.95 and 2.05 were regarded as pure RNA. In order to generate cDNA, 1 μg of high-quality RNA of each individual sample was transcribed into cDNA using thermostable RNAse inhibitor during reverse transcription

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Reviews

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Discussion

Discussion

2 years ago

Author: Paul G. Macon United States

RNA isolation from tissue

How do I extract RNA from animal tissue without using liquid nitrogen? I tried the RNA extraction by using the TRIzol reagent and I homogenize the tissue using polytron homogenizer at room temperature for 30secs is this correct?

Discussion

3 years ago

Author: Aaron Stege Netherlands

Problem in phase separation after using serum/plasma kit

I used a serum/plasma kit for my serum samples. After the phase separation the samples should have 3 phases: a colourless aqueous phase, a white interphase and a red organic phase. However, in some of my samples there was no aqueous phase unless I wait for an extended period of time. How can I circumvent this problem?

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Papers

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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for PureLink™ RNA Mini Kit below.

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