TRIzol Reagent

RNA isolation / purification Tissue - Mouse Tendons

Experiment
RNA isolation / purification Tissue - Mouse Tendons
Product
TRIzol Reagent from Thermo Fisher Scientific
Manufacturer
Thermo Fisher Scientific

Protocol tips

Protocol tips
- For low 260/230 readings the best approach is to try washing sample (precipitation) with ethanol to desalt it.

Publication protocol

Dissected Achilles tendons were placed immediately into 1.5 ml tubes containing 500 μl of TRIzol reagent (15596026; Invitrogen, Carlsbad, CA, USA) and high impact zirconium 1.5 mm beads (30–40 beads per tube, D1032-15, Benchmark, Sayreville, NJ, USA). Samples were homogenized in two 180-s rounds of bead beating at 50 Hz (BeadBug microtube homogenizer; Benchmark Scientific, Sayreville, NJ, USA). Samples were then moved directly to dry ice or −80 °C for longer storage up to six months.

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Discussion

Discussion

2 years ago

Author: Paul G. Macon United States

RNA isolation from tissue

How do I extract RNA from animal tissue without using liquid nitrogen? I tried the RNA extraction by using the TRIzol reagent and I homogenize the tissue using polytron homogenizer at room temperature for 30secs is this correct?

Discussion

3 years ago

Author: Aaron Stege Netherlands

Problem in phase separation after using serum/plasma kit

I used a serum/plasma kit for my serum samples. After the phase separation the samples should have 3 phases: a colourless aqueous phase, a white interphase and a red organic phase. However, in some of my samples there was no aqueous phase unless I wait for an extended period of time. How can I circumvent this problem?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Tissue - Mouse Tendons using TRIzol Reagent from Thermo Fisher Scientific.

Paper title
A robust method for RNA extraction and purification from a single adult mouse tendon
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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for TRIzol Reagent below.

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Videos

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