RNeasy Plus Universal Kits

RNA isolation / purification Tissue - Mouse Thymus

Experiment
RNA isolation / purification Tissue - Mouse Thymus
Product
RNeasy Plus Universal Kits from Qiagen
Manufacturer
Qiagen

Protocol tips

Upstream tips
- Kits should be stored dry at room temperature (15–25°C).
- This kit purifies RNA from up to 50 mg tissue

Publication protocol

After tissue harvesting, a fraction of the maternal liver as well as fetal liver and thymus were preserved in RNAlater (Ambion by Life Technologies GmbH) at −20°C. Tissue homogenization was performed using micropackaging vials with ceramic beads (1.4 mm) in a Precellys 24 Tissue Homogenizer (Peqlab, Erlangen, Germany). RNA isolation and DNA digestion were conducted by use of RNeasy Plus Universal Mini Kit (Qiagen, Hilden, Germany) and DNA-Free Kit (Applied Biosystems by Life Technologies GmbH), respectively. cDNA synthesis was performed with random primers (Invitrogen by Life Technologies GmbH). The concentration and purity of RNA and cDNA were assessed using NanoQuant (Tecan Group AG, Männedorf, Switzerland).

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Discussion

Discussion

4 years ago

Author: Paul G. Macon United States

RNA isolation from tissue

How do I extract RNA from animal tissue without using liquid nitrogen? I tried the RNA extraction by using the TRIzol reagent and I homogenize the tissue using polytron homogenizer at room temperature for 30secs is this correct?

Discussion

4 years ago

Author: Aaron Stege Netherlands

Problem in phase separation after using serum/plasma kit

I used a serum/plasma kit for my serum samples. After the phase separation the samples should have 3 phases: a colourless aqueous phase, a white interphase and a red organic phase. However, in some of my samples there was no aqueous phase unless I wait for an extended period of time. How can I circumvent this problem?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Tissue - Mouse Thymus using RNeasy Plus Universal Kits from Qiagen.

Paper title
Prenatal acetaminophen affects maternal immune and endocrine adaptation to pregnancy, induces placental damage, and impairs fetal development in mice.
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Manufacturer protocol

Download the product protocol from Qiagen for RNeasy Plus Universal Kits below.

Download PDF Download manufacturer protocol

Videos

Check out videos that might be relevant for performing RNA isolation / purification Tissue - Mouse Thymus using RNeasy Plus Universal Kits from Qiagen. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.

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