RNeasy Micro Kit

RNA isolation / purification Tissue - Mouse Trachea

RNA isolation / purification Tissue - Mouse Trachea
RNeasy Micro Kit from Qiagen

Protocol tips

Upstream tips
- This kit can be used for the isolation of RNA from up to 5 mg of tissue.
Downstream tips
- Always blank the spectrophotometer with your elution buffer.

- When measuring 260 nm absorbance, a unit of 1 on this scale equals to 40 ng of RNA.

Publication protocol

For mRNA quantification by RT‐qPCR in tracheas harvested at D0, D1, D3, D7, and D21, total RNA was extracted from pools of four half‐tracheas (n = 6 pools per group) with the RNAeasy Micro Kit (Qiagen) and quantified on a Nanodrop®. One μg of RNA was reverse‐transcribed with mMLV reverse transcriptase (Promega). cDNA was used for qPCR, with the Applied Biosystem primers for VEGF isoform A (Mm01281449_m1), HIF1α (Mm00468869_m1), angiopoietin‐1 (Mm00456503_m1), angiopietin‐2 (Mm00545822_m1), podoplanin (Mm00494716_m1), and HPRT1 (Mm01545399_m1) as a housekeeping gene. An ABI Prism 7000 thermocycler performed 40 cycles of qPCR for each gene. Expression of the mRNA of the gene of interest was referenced to HPRT1 (hypoxanthine phosphoribosyltransferase 1) expression (2−ΔCt) and calibrated to the D0 values (2−ΔΔCt).

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2 years ago

Author: Paul G. Macon United States

RNA isolation from tissue

How do I extract RNA from animal tissue without using liquid nitrogen? I tried the RNA extraction by using the TRIzol reagent and I homogenize the tissue using polytron homogenizer at room temperature for 30secs is this correct?


3 years ago

Author: Aaron Stege Netherlands

Problem in phase separation after using serum/plasma kit

I used a serum/plasma kit for my serum samples. After the phase separation the samples should have 3 phases: a colourless aqueous phase, a white interphase and a red organic phase. However, in some of my samples there was no aqueous phase unless I wait for an extended period of time. How can I circumvent this problem?

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Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Tissue - Mouse Trachea using RNeasy Micro Kit from Qiagen.

Paper title
Revascularization of the graft in obliterative bronchiolitis after heterotopic tracheal transplantation
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Manufacturer protocol

Download the product protocol from Qiagen for RNeasy Micro Kit below.

Download PDF Download manufacturer protocol


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