RNeasy Mini Kit

RNA isolation / purification Tissue - Mouse Vagina

RNA isolation / purification Tissue - Mouse Vagina
RNeasy Mini Kit from Qiagen

Protocol tips

Protocol tips
- To yield high RNA, give the column an extra wash with both RW1 and RPE buffers (3 total washes with each buffer).
Downstream tips
- Include DNAse treatment for 15-20min.

- Ensure EtOH is completely evaporated off of the column prior to elution. Adjust time from 1min to 5 min at 60`C.

- Use water to elute the RNA that is warmed to ~60`C.

Publication protocol

Following collection, RNA in vaginal wash fluid was stabilized by addition of 10 volumes of RNAlater solution (Qiagen, Valencia, CA), and samples were immediately processed or frozen at −80°C for later use. Total RNA was isolated from vaginal wash fluid or homogenized vaginal tissue by shaking with 0.1-mm silica spheres using Lysing Matrix B FastRNA tubes and a FastPrep high-speed reciprocating shaking device (QBioGene, Carlsbad, CA). RNA was further purified by use of an RNeasy minikit (Qiagen). Genomic DNA was removed by digestion during isolation (RNase-free DNase set; Qiagen) and by treatment with DNase I (amplification grade; Invitrogen) following spin-column purification. The integrity of the RNA was assessed by electrophoresis, and purity was measured by determination of the A260/A280 ratio (purity was acceptable if it was >1.8).

Full paper   Login or join for free to view the full paper.


RNeasy Mini Kit from Qiagen has not yet been reviewed for this experiment

We'd love it if you would be the first to write a review!



2 years ago

Author: Paul G. Macon United States

RNA isolation from tissue

How do I extract RNA from animal tissue without using liquid nitrogen? I tried the RNA extraction by using the TRIzol reagent and I homogenize the tissue using polytron homogenizer at room temperature for 30secs is this correct?


3 years ago

Author: Aaron Stege Netherlands

Problem in phase separation after using serum/plasma kit

I used a serum/plasma kit for my serum samples. After the phase separation the samples should have 3 phases: a colourless aqueous phase, a white interphase and a red organic phase. However, in some of my samples there was no aqueous phase unless I wait for an extended period of time. How can I circumvent this problem?

Share your thoughts or question with experts in your field by adding a discussion!


Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Tissue - Mouse Vagina using RNeasy Mini Kit from Qiagen.

Paper title
Murine Vaginal Colonization Model for Investigating Asymptomatic Mucosal Carriage of
Full paper
Login or join for free to view the full paper.

Manufacturer protocol

Download the product protocol from Qiagen for RNeasy Mini Kit below.

Download PDF Download manufacturer protocol


Check out videos that might be relevant for performing RNA isolation / purification Tissue - Mouse Vagina using RNeasy Mini Kit from Qiagen. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.

We haven't found any additional videos for this experiment / product combination yet.

Outsource your experiment

Fill out your contact details and receive price quotes in your Inbox

  Outsource experiment
Become shareholder Discussions About us Contact Privacy Terms