TRIzol Reagent

RNA isolation / purification Cells - primary bovine pulmonary artery smooth muscle cells

Experiment
RNA isolation / purification Cells - primary bovine pulmonary artery smooth muscle cells
Product
TRIzol Reagent from Thermo Fisher Scientific
Manufacturer
Thermo Fisher Scientific

Protocol tips

Protocol tips
- For low 260/230 readings the best approach is to try washing sample (precipitation) with ethanol to desalt it.

Publication protocol

Total RNA was extracted from cells by use of TRIzol reagent and reverse transcription was performed by using a High-Capacity cDNA Reverse Transcription Kit according to manufacturer's instructions (Life Technologies).

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Reviews

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Discussion

Discussion

2 years ago

Author: Switzerland

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

Share your thoughts or question with experts in your field by adding a discussion!

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Cells - primary bovine pulmonary artery smooth muscle cells using TRIzol Reagent from Thermo Fisher Scientific.

Paper title
Role of hypoxia-induced transglutaminase 2 in pulmonary artery smooth muscle cell proliferation
Full paper
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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for TRIzol Reagent below.

Download PDF Download manufacturer protocol

Videos

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