RNeasy Mini Kit

RNA isolation / purification Cells - primary bovine pulmonary artery smooth muscle cells

Experiment
RNA isolation / purification Cells - primary bovine pulmonary artery smooth muscle cells
Product
RNeasy Mini Kit from Qiagen
Manufacturer
Qiagen

Protocol tips

Protocol tips
- To yield high RNA, give the column an extra wash with both RW1 and RPE buffers (3 total washes with each buffer).
Downstream tips
- Include DNAse treatment for 15-20min.

- Ensure EtOH is completely evaporated off of the column prior to elution. Adjust time from 1min to 5 min at 60`C.

- Use water to elute the RNA that is warmed to ~60`C.

Publication protocol

Total RNA from cells was obtained using the RNeasy kit (Qiagen, Hilden, Germany) following the manufacturer's instructions treated with the DNAse 1, amplification grade (Sigma, St Louis, MO), to eliminate the genome DNA contamination and quantified using the NanoDrop ND-1000 Spectrophotometer (Nano-Drop Technologies, Wilmington, DE). Absorbance of the RNA samples was quantified at 260 and 280 nm, and the 260/280 ratio was calculated. The samples showed a 260/280 ratio ≥1.9, which was assumed as an indicator of RNA purity.

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Discussion

Discussion

2 years ago

Author: Switzerland

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Cells - primary bovine pulmonary artery smooth muscle cells using RNeasy Mini Kit from Qiagen.

Paper title
Gene Therapy by Targeted Adenovirus-mediated Knockdown of Pulmonary Endothelial Tph1 Attenuates Hypoxia-induced Pulmonary Hypertension
Full paper
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Manufacturer protocol

Download the product protocol from Qiagen for RNeasy Mini Kit below.

Download PDF Download manufacturer protocol

Videos

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