RNeasy Mini Kit

RNA isolation / purification Cells - primary human hair follicle dermal papilla cells

Experiment
RNA isolation / purification Cells - primary human hair follicle dermal papilla cells
Product
RNeasy Mini Kit from Qiagen
Manufacturer
Qiagen

Protocol tips

Protocol tips
- To yield high RNA, give the column an extra wash with both RW1 and RPE buffers (3 total washes with each buffer).
Downstream tips
- Include DNAse treatment for 15-20min.

- Ensure EtOH is completely evaporated off of the column prior to elution. Adjust time from 1min to 5 min at 60`C.

- Use water to elute the RNA that is warmed to ~60`C.

Publication protocol

Total RNA was extracted from the cells using the RNeasy Mini kit (Qiagen Inc., Valencia, CA, USA) as described previously11. Briefly, the cells were lysed and homogenized in RLT buffer supplemented with 10 ┬Ál/ml mercaptoethanol (Sigma-Aldrich, Munich, Germany). The lysate was homogenized using a syringe and a 20-G needle. The sample was placed in a silica column followed by washing and eluting with RNase-free water. The RNA concentration was measured using an ultraviolet spectrophotometry at 260 nm, and the purity and integrity were evaluated using the A260/A280 ratio.

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Discussion

Discussion

1 year ago

Author: Switzerland

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Cells - primary human hair follicle dermal papilla cells using RNeasy Mini Kit from Qiagen.

Paper title
Herbal Extracts Induce Dermal Papilla Cell Proliferation of Human Hair Follicles
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Manufacturer protocol

Download the product protocol from Qiagen for RNeasy Mini Kit below.

Download PDF Download manufacturer protocol

Videos

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