RNeasy Plus Mini Kit

RNA isolation / purification Cells - primary human carotid artery endothelial cells

Experiment
RNA isolation / purification Cells - primary human carotid artery endothelial cells
Product
RNeasy Plus Mini Kit from Qiagen
Manufacturer
Qiagen

Protocol tips

Downstream tips
- Include DNAse treatment for 15-20min.

- Ensure EtOH is completely evaporated off of the column prior to elution. Adjust time from 1min to 5 min at 60`C.

- Use water to elute the RNA that is warmed to ~60`C.

Publication protocol

Total RNA was prepared from HCtAECs using an RNeasy Plus Mini Kit (QIAGEN, Hilden, Germany), and transcribed to cDNA using a High‐Capacity cDNA Reverse Transcription Kit (Life Technologies Corporation, CA).

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Reviews

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Discussion

Discussion

2 years ago

Author: Switzerland

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

Share your thoughts or question with experts in your field by adding a discussion!

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Cells - primary human carotid artery endothelial cells using RNeasy Plus Mini Kit from Qiagen.

Paper title
A sphingosine‐1‐phosphate receptor type 1 agonist, ASP4058, suppresses intracranial aneurysm through promoting endothelial integrity and blocking macrophage transmigration
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Manufacturer protocol

Download the product protocol from Qiagen for RNeasy Plus Mini Kit below.

Download PDF Download manufacturer protocol

Videos

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