RNeasy Mini Kit

RNA isolation / purification Cells - primary human chondrocytes - osteoarthritis

Experiment
RNA isolation / purification Cells - primary human chondrocytes - osteoarthritis
Product
RNeasy Mini Kit from Qiagen
Manufacturer
Qiagen

Protocol tips

Protocol tips
- To yield high RNA, give the column an extra wash with both RW1 and RPE buffers (3 total washes with each buffer).
Downstream tips
- Include DNAse treatment for 15-20min.

- Ensure EtOH is completely evaporated off of the column prior to elution. Adjust time from 1min to 5 min at 60`C.

- Use water to elute the RNA that is warmed to ~60`C.

Publication protocol

Total RNA was isolated using Qiagen RNeasy Mini Kit (Qiagen, Valencia, CA, USA) on Qiacube automated sample prep platform (Qiagen) according to the manufacturer’s protocol. Total RNA concentration was determined by absorbance at 260 nm using the Eppendorf spectrophotometer.

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Reviews

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Discussion

Discussion

2 years ago

Author: Switzerland

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Cells - primary human chondrocytes - osteoarthritis using RNeasy Mini Kit from Qiagen.

Paper title
Delphinidin inhibits IL-1β-induced activation of NF-κB by modulating the phosphorylation of IRAK-1 in human articular chondrocytes
Full paper
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Manufacturer protocol

Download the product protocol from Qiagen for RNeasy Mini Kit below.

Download PDF Download manufacturer protocol

Videos

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