RNeasy Mini Kit

RNA isolation / purification Cells - primary human osteoblasts

Experiment
RNA isolation / purification Cells - primary human osteoblasts
Product
RNeasy Mini Kit from Qiagen
Manufacturer
Qiagen

Protocol tips

Protocol tips
- To yield high RNA, give the column an extra wash with both RW1 and RPE buffers (3 total washes with each buffer).
Downstream tips
- Include DNAse treatment for 15-20min.

- Ensure EtOH is completely evaporated off of the column prior to elution. Adjust time from 1min to 5 min at 60`C.

- Use water to elute the RNA that is warmed to ~60`C.

Publication protocol

Total RNA was isolated from HOB using the RNeasy RNA isolation kit (Qiagen,Valencia, CA) with quality assessed by electrophoretic analysis on an Agilent Model 2100 Bioanalyzer. RNA samples had integrity numbers greater than 8.0 (8.4–10). RNA (250 ng) was used as the template for synthesis of internally labeled cRNAs using the Agilent QuickAmp Labeling kit and cyanine 3-CTP and cyanine 5-CTP (Perkin Elmer, Waltham, MA) and a modified QuickAmp protocol .

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Reviews

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Discussion

Discussion

1 year ago

Author: Switzerland

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

Share your thoughts or question with experts in your field by adding a discussion!

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Cells - primary human osteoblasts using RNeasy Mini Kit from Qiagen.

Paper title
Bone Marrow Osteoblast Damage by Chemotherapeutic Agents
Full paper
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Manufacturer protocol

Download the product protocol from Qiagen for RNeasy Mini Kit below.

Download PDF Download manufacturer protocol

Videos

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