5/5 - 1 Review - 100% recommends this
|- To yield high RNA, give the column an extra wash with both RW1 and RPE buffers (3 total washes with each buffer).|
|- Include DNAse treatment for 15-20min.
- Ensure EtOH is completely evaporated off of the column prior to elution. Adjust time from 1min to 5 min at 60`C.
- Use water to elute the RNA that is warmed to ~60`C.
2 years ago
I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?
Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Cells - primary human placental epithelial cells using RNeasy Mini Kit from Qiagen.
Download the product protocol from Qiagen for RNeasy Mini Kit below.Download manufacturer protocol
Check out videos that might be relevant for performing RNA isolation / purification Cells - primary human placental epithelial cells using RNeasy Mini Kit from Qiagen. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.
We haven't found any additional videos for this experiment / product combination yet.