TriPure Isolation Reagent

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	- For low contamination rate carefully remove the upper aqueous phase - Adding too little reagent can lead to contamination with DNA

Protocol tips
- For low contamination rate carefully remove the upper aqueous phase - Adding too little reagent can lead to contamination with DNA

Publication protocol

Total RNA was isolated using TriPure (Roche) according to the manufacturer's protocol. DNAse (DNA-free, Life Technologies)-treated RNA was reverse transcribed into complementary DNA using iScript (BioRad). Quantitative PCR analyses were performed using SYBR green (iQ SYBR Green Supermix, BioRad) on BioRad CFX97. Primer sequences used for qRT–PCR analyses were listed in Supplementary Table 2. HUGO Gene Nomenclature Committee's Guideline was used for gene names written in this manuscript.

Full paper   Login or join for free to view the full paper.

Reviews

Discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Cells - primary human preadipocytes using TriPure Isolation Reagent from Sigma-Aldrich.

Manufacturer protocol

Download the product protocol from Sigma-Aldrich for TriPure Isolation Reagent below.

Download manufacturer protocol

Videos

Check out videos that might be relevant for performing RNA isolation / purification Cells - primary human preadipocytes using TriPure Isolation Reagent from Sigma-Aldrich. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.