RNeasy Mini Kit

RNA isolation / purification Cells - primary porcine coronary artery endothelial cells

Experiment
RNA isolation / purification Cells - primary porcine coronary artery endothelial cells
Product
RNeasy Mini Kit from Qiagen
Manufacturer
Qiagen

Protocol tips

Protocol tips
- To yield high RNA, give the column an extra wash with both RW1 and RPE buffers (3 total washes with each buffer).
Downstream tips
- Include DNAse treatment for 15-20min.

- Ensure EtOH is completely evaporated off of the column prior to elution. Adjust time from 1min to 5 min at 60`C.

- Use water to elute the RNA that is warmed to ~60`C.

Publication protocol

Primary cultures (cells at passage zero) were used for microarray and RT-PCR analysis. Briefly, native and regenerated endothelial cells were cultured for 10 days to reach ∼80% confluence before RNA isolation using a RNeasy Mini kit (Qiagen, Valencia, CA) (20). The yield of RNA from each sample ranged from 600 ng to 17 μg. The ratio of absorption (260/280) of all samples was between 1.7 and 2.1. The integrity of RNA samples was assessed qualitatively (rRNA ratio for 28s/18s between 1.9 and 2.1). Samples were stored immediately at −70°C until use.

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Discussion

Discussion

4 years ago

Author: Switzerland

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

Share your thoughts or question with experts in your field by adding a discussion!

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Cells - primary porcine coronary artery endothelial cells using RNeasy Mini Kit from Qiagen.

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Manufacturer protocol

Download the product protocol from Qiagen for RNeasy Mini Kit below.

Download PDF Download manufacturer protocol

Videos

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