TRIzol Reagent

RNA isolation / purification Cells - primary porcine coronary artery endothelial cells

Experiment
RNA isolation / purification Cells - primary porcine coronary artery endothelial cells
Product
TRIzol Reagent from Thermo Fisher Scientific
Manufacturer
Thermo Fisher Scientific

Protocol tips

Protocol tips
- For low 260/230 readings the best approach is to try washing sample (precipitation) with ethanol to desalt it.

Publication protocol

To determine the mRNA expression of ICAM-1, the total RNA was isolated from cells using TRIzol reagent (Invitrogen; Thermo Fisher Scientific, Inc., Waltham, MA, USA), according to the manufacturer's protocol. RNA was frozen at −80°C until analyses were performed. Any DNA contamination in the isolated RNA was eliminated with DNase treatment. The RNA concentration was determined spectrophotometrically at 260 nm (SmartSpec™ 3000; Bio-Rad Laboratories, Inc., Hercules, CA, USA). The RNA integrity was evaluated by 1% agarose gel electrophoresis. Of the total RNA, 3 µg was used for the RT reaction using the ThermoScript RT-PCR systems (Invitrogen; Thermo Fisher Scientific, Inc.), according to the manufacturer's protocols.

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Discussion

Discussion

2 years ago

Author: Switzerland

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Cells - primary porcine coronary artery endothelial cells using TRIzol Reagent from Thermo Fisher Scientific.

Paper title
Morphine-induced delayed pre-conditioning against anoxia/reoxygenation injury in pulmonary artery endothelial cells: The role of mitochondrial KATP channels.
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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for TRIzol Reagent below.

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