miRNeasy Micro Kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
- This kit can be used for the isolation of RNA from up to 1x10^6 cells

Upstream tips
- This kit can be used for the isolation of RNA from up to 1x10^6 cells

Publication protocol

RNA was isolated using the miRNAeasy kit (Qiagen, Germantown, MD), according to the manufacturer's manual, which allows for the preservation of both the small RNA and mRNA fraction. RNA purity was determined using the Nanodrop ND1000 (Thermo Scientific, Waltham, MA) UV-spectrophotometer. The 260/280nm ratios were measured and samples with ratios of 2.0 ± 0.05 were considered pure. This was followed by RNA integrity assessment with a 1% agarose gel imaged with the Gel doc XR system (Bio-Rad, Hercules, CA). Samples showing clearly visible S28 and S18 rRNA bands were considered to be intact.

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Manufacturer protocol

Download the product protocol from Qiagen for miRNeasy Micro Kit below.

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