NucleoSpin® RNA

RNA isolation / purification Cells - primary rat dermal fibroblasts

Experiment
RNA isolation / purification Cells - primary rat dermal fibroblasts
Product
NucleoSpin® RNA from Macherey Nagel
Manufacturer
Macherey Nagel

Protocol tips

Upstream tips
- Aliquot rDNase and store at -20 °C.
Protocol tips
Dry the columns very well after the ethanol wash by adding an additional 2' >10000 rpm centrifuge with no buffer.

Publication protocol

RNA was isolated with NucleoSpin®RNA II kit (Macherey-Nagel, Duren, Germany), according to manufacturer's protocol. The quantity and quality of the isolated total RNA was evaluated spectrophotometrically using ND-2000-Spectrophotometer NanoDrop 2000 with software for analysis of nucleic acids (Thermo Fisher Scientific, Wilmington, Delaware, USA).

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Reviews

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Discussion

Discussion

4 years ago

Author: Switzerland

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Cells - primary rat dermal fibroblasts using NucleoSpin® RNA from Macherey Nagel.

Paper title
Influence of cartilage interstitial fluid on gene expression in cruciate ligament fibroblasts
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Manufacturer protocol

Download the product protocol from Macherey Nagel for NucleoSpin® RNA below.

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Videos

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