FITC Annexin V Apoptosis Detection Kit I

Apoptosis assay cell type - lymphoblastic leukaemia

Experiment
Apoptosis assay cell type - lymphoblastic leukaemia
Product
FITC Annexin V Apoptosis Detection Kit I from BD Biosciences
Manufacturer
BD Biosciences

Protocol tips

Protocol tips
Cell death was assayed by phosphatidylserine externalization, investigated by annexin V (FITC or APC-conjugated) staining and analysis of the DNA content by propidium iodide (PI) incorporation, performed as previously described (34).

Publication protocol

Cell death was assayed by phosphatidylserine externalization, investigated by annexin V (FITC or APC-conjugated) staining and analysis of the DNA content by propidium iodide (PI) incorporation, performed as previously described (34). For cell cycle analysis MEC1 clones were harvested, fixed in cold 70% ethanol, added drop-wise to the pellet while vortexing to minimize clumping. After 2 h at −20 °C, cells were washed, RNase treated and stained with PI. All samples were analysed by a FACScan (Becton Dickinson, BD), flow cytometer and/or a C6 BDAccuri flow cytometer (BD). For PI assays, clumps and doublets were excluded from the analysis and DNA content of the nuclei was registered on a logarithmic or linear scale for apoptosis and cell cycle, respectively.


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