PureLink™ RNA Mini Kit

RNA isolation / purification Cells - primary mouse cortical neurons

Experiment
RNA isolation / purification Cells - primary mouse cortical neurons
Product
PureLink™ RNA Mini Kit from Thermo Fisher Scientific
Manufacturer
Thermo Fisher Scientific

Protocol tips

Protocol tips
- Depending on the material you want to isolate RNA from, please refer to the manual for additional reagents/lab equipment that are required
Downstream tips
- DNase is not supplied with this kit, a DNase kit is available which is compatible with this kit

Publication protocol

RNA can be extracted using method of choice. We use invitrogen purelink kit with modifications Modification: Invitrogen recommends that equal volume of 100%
Ethanol be added to aqueous phase to get a final concentration of 50% Ethanol. We find that increasing final concentration to 70% increases the short RNA and therefore the total yield of the sample

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Reviews

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Discussion

Discussion

2 years ago

Author: Switzerland

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Cells - primary mouse cortical neurons using PureLink™ RNA Mini Kit from Thermo Fisher Scientific.

Paper title
Trehalose-enhanced isolation of neuronal sub-types from adult mouse brain
Full paper
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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for PureLink™ RNA Mini Kit below.

Download PDF Download manufacturer protocol

Videos

Check out videos that might be relevant for performing RNA isolation / purification Cells - primary mouse cortical neurons using PureLink™ RNA Mini Kit from Thermo Fisher Scientific. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.

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