RNeasy Micro Kit

RNA isolation / purification Cells - primary mouse dorsal root ganglion neurons

Experiment
RNA isolation / purification Cells - primary mouse dorsal root ganglion neurons
Product
RNeasy Micro Kit from Qiagen
Manufacturer
Qiagen

Protocol tips

Upstream tips
- This kit can be used for the isolation of RNA from up to 5x10^5 cells
Downstream tips
- Always blank the spectrophotometer with your elution buffer.

- When measuring 260 nm absorbance, a unit of 1 on this scale equals to 40 ng of RNA.

Publication protocol

Animals were deeply anesthetized with pentobarbital and transcardially perfused with PBS. DRG (lumbar, L3–L6) and TG were collected and RNA was prepared with the RNeasy Micro kit (Qiagen). Total RNA (500 μg) was converted to cDNA using the SuperScript III Reverse Transcriptase kit (Thermo Fisher Scientific).

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Discussion

Discussion

2 years ago

Author: Switzerland

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

Share your thoughts or question with experts in your field by adding a discussion!

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Cells - primary mouse dorsal root ganglion neurons using RNeasy Micro Kit from Qiagen.

Paper title
The Molecular Fingerprint of Dorsal Root and Trigeminal Ganglion Neurons
Full paper
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Manufacturer protocol

Download the product protocol from Qiagen for RNeasy Micro Kit below.

Download PDF Download manufacturer protocol

Videos

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