|- Incubate at 80°C for 5 minutes to lyse the cells; then cool to room temperature.
- Add 3µl of RNase Solution to the cell lysate. Invert the tube 2–5 times to mix.
- Incubate at 37°C for 15–60 minutes. Cool the sample to room temperature
|Rehydrate DNA by incubating 1 hour at 65°C, and then leave the sample at room temperature or 4°C overnight if DNA pellet difficult to dissolve|
Wizard® Genomic DNA Purification Kit from Promega has not yet been reviewed for this experiment
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2 years ago
The DNA concentration after using this DNA isolation kit is sometimes too low and thus it is not sufficient for my follow-up experiments. How can I improve it?
2 years ago
Author: Milena Alexeyeva
Hello there! I just started doing experiments on bacterial DNA and I would like your opinion on storing DNA templates. Which are the desired and most optimal conditions?
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