TRIzol Reagent

RNA isolation / purification Tissue - Rat Epididymis

Experiment
RNA isolation / purification Tissue - Rat Epididymis
Product
TRIzol Reagent from Thermo Fisher Scientific
Manufacturer
Thermo Fisher Scientific

Protocol tips

Protocol tips
- For low 260/230 readings the best approach is to try washing sample (precipitation) with ethanol to desalt it.

Publication protocol

Total RNA was extracted from the rat epididymal fat tissue using TRIzol Reagent (Invitrogen, Carlsbad, CA, USA) according to the instructions provided by the manufacturer. Total RNA was subjected to reverse transcription in a 20 µL reaction containing random primers and SuperscriptII enzyme (Invitrogen). Quantitative real-time PCR was performed with an ABI Prism 7700 Sequence Detection System using SYBR® Green PCR Master Mix Reagent Kit (Applied Biosystems, Foster City, CA, USA)

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Discussion

Discussion

1 year ago

Author: Paul G. Macon United States

RNA isolation from tissue

How do I extract RNA from animal tissue without using liquid nitrogen? I tried the RNA extraction by using the TRIzol reagent and I homogenize the tissue using polytron homogenizer at room temperature for 30secs is this correct?

Discussion

2 years ago

Author: Aaron Stege Netherlands

Problem in phase separation after using serum/plasma kit

I used a serum/plasma kit for my serum samples. After the phase separation the samples should have 3 phases: a colourless aqueous phase, a white interphase and a red organic phase. However, in some of my samples there was no aqueous phase unless I wait for an extended period of time. How can I circumvent this problem?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Tissue - Rat Epididymis using TRIzol Reagent from Thermo Fisher Scientific.

Paper title
The role of adipose tissue asymmetric dimethylarginine/dimethylarginine dimethylaminohydrolase pathway in adipose tissue phenotype and metabolic abnormalities in subtotally nephrectomized rats.
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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for TRIzol Reagent below.

Download PDF Download manufacturer protocol

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