RNeasy Mini Kit

RNA isolation / purification Tissue - Rat Jejunum

RNA isolation / purification Tissue - Rat Jejunum
RNeasy Mini Kit from Qiagen

Protocol tips

Protocol tips
- To yield high RNA, give the column an extra wash with both RW1 and RPE buffers (3 total washes with each buffer).
Downstream tips
- Include DNAse treatment for 15-20min.

- Ensure EtOH is completely evaporated off of the column prior to elution. Adjust time from 1min to 5 min at 60`C.

- Use water to elute the RNA that is warmed to ~60`C.

Publication protocol

The hypothalamus was quickly dissected from a semi-frozen brain. Total RNA was isolated from the hypothalamus, duodenum, jejunum, and ileum using RNeasy mini-columns (Qiagen, Valencia, CA). Genomic DNA was eliminated by DNase I on column treatment with RNase-free DNase set (Qiagen, Valencia, CA). RNA integrity was confirmed by visualizing an approximate 2/1 ratio of 28S to 18S band on the 1% agarose gel stained by ethidium bromide. RNA purity was confirmed with absorbance ratio of >2.0 at 260nm/280nm using NanoVue (GE Healthcare, Piscataway, NJ). 1 μg of total RNA was used to generate cDNA using iScript following manufacturer's protocol (Bio-Rad, Hercules, CA). cDNA was made from all samples for each tissue at the same time to minimize experimental variations.

Full paper   Login or join for free to view the full paper.


RNeasy Mini Kit from Qiagen has not yet been reviewed for this experiment

We'd love it if you would be the first to write a review!



1 year ago

Author: Paul G. Macon United States

RNA isolation from tissue

How do I extract RNA from animal tissue without using liquid nitrogen? I tried the RNA extraction by using the TRIzol reagent and I homogenize the tissue using polytron homogenizer at room temperature for 30secs is this correct?


2 years ago

Author: Aaron Stege Netherlands

Problem in phase separation after using serum/plasma kit

I used a serum/plasma kit for my serum samples. After the phase separation the samples should have 3 phases: a colourless aqueous phase, a white interphase and a red organic phase. However, in some of my samples there was no aqueous phase unless I wait for an extended period of time. How can I circumvent this problem?

Share your thoughts or question with experts in your field by adding a discussion!


Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Tissue - Rat Jejunum using RNeasy Mini Kit from Qiagen.

View full paper   Login or join for free to view the full paper.

Manufacturer protocol

Download the product protocol from Qiagen for RNeasy Mini Kit below.

Download PDF Download manufacturer protocol


Check out videos that might be relevant for performing RNA isolation / purification Tissue - Rat Jejunum using RNeasy Mini Kit from Qiagen. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.

We haven't found any additional videos for this experiment / product combination yet.

Become shareholder Discussions About us Contact Privacy Terms