High Pure RNA Isolation Kit

RNA isolation / purification Tissue - Rat Kidney

Experiment
RNA isolation / purification Tissue - Rat Kidney
Product
High Pure RNA Isolation Kit from Roche Lifesciences
Manufacturer
Roche Lifesciences

Protocol tips

Upstream tips
- To decrease RNase activity,process starting material immeditely or store at 80C.

- To increase nucleic yield or purity store all buffer at +15C to +25C.
Protocol tips
- To decrease RNase activity,use eluted RNA directly in downstream procedure or store at -80C immediately

Publication protocol

Kidney tissues snap frozen and stored at -80°C were used for RNA isolation. Tissue piece (100 mg) was transferred to a DNA/RNA-free round bottom tube containing CHAPS buffer (Cat. No. 90436, Millipore Co., CA) and gently homogenized (TEKMAR Tissuemizer Co., Cincinnati, OH) for 10–20 s (seconds). The homogenized tissue was kept in ice for 20 min followed by centrifugation (12,000 x g for 20 min). The supernatant (200 μL) was mixed with 400 μL of RNA lysis buffer (High Pure RNA Isolation kit, Cat. No.11 828 665001, Roche Diagnostics, Mannheim, Germany) and the procedure was continued as per manufacturer’s instructions. The isolated RNA was assessed for purity and quantity by spectrophotometry (Nanodrop ND-100, Nanodrop technologies, Inc., Wilmington, DE). A ratio >2 OD @ 260 nm/280 nm was considered high quality RNA for further studies.

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Discussion

Discussion

1 year ago

Author: Paul G. Macon United States

RNA isolation from tissue

How do I extract RNA from animal tissue without using liquid nitrogen? I tried the RNA extraction by using the TRIzol reagent and I homogenize the tissue using polytron homogenizer at room temperature for 30secs is this correct?

Discussion

2 years ago

Author: Aaron Stege Netherlands

Problem in phase separation after using serum/plasma kit

I used a serum/plasma kit for my serum samples. After the phase separation the samples should have 3 phases: a colourless aqueous phase, a white interphase and a red organic phase. However, in some of my samples there was no aqueous phase unless I wait for an extended period of time. How can I circumvent this problem?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Tissue - Rat Kidney using High Pure RNA Isolation Kit from Roche Lifesciences.

Paper title
Effects of Valproic Acid and Dexamethasone Administration on Early Bio-Markers and Gene Expression Profile in Acute Kidney Ischemia-Reperfusion Injury in the Rat
Full paper
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Manufacturer protocol

Download the product protocol from Roche Lifesciences for High Pure RNA Isolation Kit below.

Download PDF Download manufacturer protocol

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