RNAGEM™ Tissue PLUS

RNA isolation / purification Tissue - Rat Pineal gland

Experiment
RNA isolation / purification Tissue - Rat Pineal gland
Product
RNAGEM™ Tissue PLUS from ZyGEM
Manufacturer
ZyGEM

Protocol tips

Downstream tips
- Absorbance 260/280 nm is an ineffective quantitation method with RNAGEM-prepared nucleic acids

Publication protocol

For qRT-PCR analysis of the effect of Crx-sh on Aanat mRNA levels in cultured rat pinealocytes, pinealocyte samples were centrifuged and total RNA was extracted using RNAGEMM Tissue (ZyGEM) according to the instruction of the manufacturer.

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Reviews

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Discussion

Discussion

4 years ago

Author: Paul G. Macon United States

RNA isolation from tissue

How do I extract RNA from animal tissue without using liquid nitrogen? I tried the RNA extraction by using the TRIzol reagent and I homogenize the tissue using polytron homogenizer at room temperature for 30secs is this correct?

Discussion

4 years ago

Author: Aaron Stege Netherlands

Problem in phase separation after using serum/plasma kit

I used a serum/plasma kit for my serum samples. After the phase separation the samples should have 3 phases: a colourless aqueous phase, a white interphase and a red organic phase. However, in some of my samples there was no aqueous phase unless I wait for an extended period of time. How can I circumvent this problem?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Tissue - Rat Pineal gland using RNAGEM™ Tissue PLUS from ZyGEM.

Paper title
Circadian dynamics of the cone-rod homeobox (CRX) transcription factor in the rat pineal gland and its role in regulation of arylalkylamine N-acetyltransferase (AANAT).
Full paper
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Manufacturer protocol

Download the product protocol from ZyGEM for RNAGEM™ Tissue PLUS below.

Download PDF Download manufacturer protocol

Videos

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