NucleoSpin® RNA

Protocol tips

Upstream tips	Protocol tips	Downstream tips
- Aliquot rDNase and store at -20 °C.	- Dry the columns very well after the ethanol wash by adding an additional 2' >10000 rpm centrifuge with no buffer.

Upstream tips
- Aliquot rDNase and store at -20 °C.
Protocol tips
- Dry the columns very well after the ethanol wash by adding an additional 2' >10000 rpm centrifuge with no buffer.

Publication protocol

The organs were homogenized using a RNA lysis buffer (Macherey-Nagel) and genomic DNA was isolated from each tissue (25 mg ± 5 mg, each) or 0.2 ml of blood by using the NucleoSpin RNA II kit followed by NucleoSpin RNA/DNA buffer set (Macherey-Nagel). Genomic DNA from the rat blood was isolated using the NucleoSpin Blood kit (Macherey-Nagel). The quality of extracted DNA was examined by amplifying rat genomic GAPDH using a QPCR assay.

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Papers

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Manufacturer protocol

Download the product protocol from Macherey Nagel for NucleoSpin® RNA below.

Videos

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