TRI Reagent® MRC

RNA isolation / purification Yeast - Coprinus cinereus

Experiment
RNA isolation / purification Yeast - Coprinus cinereus
Product
TRI Reagent® MRC from Molecular Research Center, Inc.
Manufacturer
Molecular Research Center, Inc.

Protocol tips

Protocol tips
Total RNA was extracted by TRI® reagent (Molecular Research Center, Inc) and Poly(A)+ mRNA was furthered isolated using the PolyATract® mRNA isolation system (Promega).

Publication protocol

Total RNA was extracted by TRI® reagent (Molecular Research Center, Inc) and Poly(A)+ mRNA was furthered isolated using the PolyATract® mRNA isolation system (Promega). First strand cDNA was synthesized using SuperScript™ III First-Strand Synthesis System for qRT-PCR (Invitrogen). Two different template switching oligos were employed for each developmental stage. Reverse transcription was performed at 42°C for 90 minutes. Second strand cDNA was then synthesized by low cycle primer extension using Advantage® 2 polymerase (Clontech). Double-stranded cDNA was purified by QIAquick PCR purification kit (Qiagen) and checked by electrophoresis on a 1.5% agarose gel.

Full paper   Login or join for free to view the full paper.

Reviews

TRI Reagent® MRC from Molecular Research Center, Inc. has not yet been reviewed for this experiment

We'd love it if you would be the first to write a review!

Discussion

Start your discussion

Share your thoughts or question with experts in your field

Start a discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Yeast - Coprinus cinereus using TRI Reagent® MRC from Molecular Research Center, Inc..

Paper title
5'-Serial Analysis of Gene Expression studies reveal a transcriptomic switch during fruiting body development in
Full paper
Login or join for free to view the full paper.

Manufacturer protocol

Download the product protocol from Molecular Research Center, Inc. for TRI Reagent® MRC below.

Download PDF Download manufacturer protocol

Videos

Check out videos that might be relevant for performing RNA isolation / purification Yeast - Coprinus cinereus using TRI Reagent® MRC from Molecular Research Center, Inc.. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.

We haven't found any additional videos for this experiment / product combination yet.

Outsource your experiment

Fill out your contact details and receive price quotes in your Inbox

  Outsource experiment
Become shareholder Discussions About us Contact Privacy Terms