TRIzol Reagent

RNA isolation / purification Yeast - Schizophyllum commune

Experiment
RNA isolation / purification Yeast - Schizophyllum commune
Product
TRIzol Reagent from Thermo Fisher Scientific
Manufacturer
Thermo Fisher Scientific

Protocol tips

Protocol tips
Dry the columns very well after the ethanol wash by adding an additional 2' >10000 rpm centrifuge with no buffer.

Publication protocol

RNA was extracted using TriZol (Life technologies, Carlsbad, USA) and purified using the NucleoSpin RNA kit (Macherey-Nagel, Düren, Germany). Quality of RNA was checked using the BioAnalyzer and sent to ServiceXS (Leiden, the Netherlands) for Illumina Next Generation Sequencing. RNAseq data have been deposited at NCBI under BioProject PRJNA323434.

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Manufacturer protocol

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